Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.

From the reviews:

"Ever wondered how to catch your FISH ? in the dark pools of cellular biology? Then ? FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) ? . Every chapter is illustrated by several schematic drawings ? . The manual is completed by a subject index ? . The book is certainly clear ? providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)