Biophysical chemistry is a multidisciplinary field that uses models of chemistry and physics to comprehend organic systems by interpreting the quantitative, energetics, structural, functional and interactional context of the physical nature. In this introductory volume, the authors provide insight into the significance of biophysics chemistry as a developing area of science and the wide variety of strategies that have been applied to explain the related phenomena. The book discusses a few specific methods for reviewing biological molecules and how they function. A few of these procedures use environmental and semi-environmental factors as that for organic particles in the natural environment. The book focuses on different methods that up to date are utilized in biophysical chemistry. Every method comes with a brief definition and is cited with proper reference for future research.